Comprehensive enzyme characterization through advanced activity assays. From kinetic profiling to high-throughput screening, we deliver precise quantitative data to support your research and development programs.
Our enzyme activity assay platform combines automated liquid handling with advanced detection methods to deliver high-quality kinetic data across a wide range of enzyme classes.
Complete kinetic characterization including Michaelis-Menten analysis, inhibition studies, and mechanism determination for all enzyme classes.
High-throughput screening of substrate panels to determine specificity, selectivity, and optimal reaction conditions.
Comprehensive selectivity profiling including enantioselectivity, regioselectivity, and chemoselectivity for biocatalytic applications.
From basic activity screening to advanced mechanism studies, our platform covers all aspects of enzyme characterization.
Comprehensive kinetic profiling to determine catalytic parameters including Km, Vmax, kcat, and Ki for inhibitors.
Thermal stability profiling using DSF, nanoDSF, and differential scanning calorimetry for process conditions.
High-throughput screening of compound libraries with IC50 determination and selectivity profiling.
Detailed mechanistic analysis including pH-rate profiles, isotope effects, and dead-end inhibition patterns.
Enginoma combines cutting-edge technology with expert scientific guidance to deliver reliable enzyme characterization data.
96-well and 384-well format assays with automated liquid handling for rapid screening of hundreds of conditions.
Fluorescence, absorbance, luminescence, and mass spectrometry detection for diverse enzyme classes.
All assays follow SOPs with appropriate controls and statistical rigor for reproducible results.
Dedicated scientific consultation to design optimal experimental approaches for your enzyme system.
Our enzyme assay services support research across multiple fields and industries.
Kinase and protease inhibitor profiling for pharmaceutical development. IC50 determination and selectivity panels against off-target enzymes.
Characterization of lipases, cellulases, and amylases for detergent, food, and textile applications. Performance profiling under process conditions.
Enantioselectivity determination for asymmetric synthesis. E-value measurement and substrate scope mapping for stereoselective transformations.
Our proven workflow ensures efficient project delivery with clear communication at every stage.
Project scope discussion, assay feasibility assessment, and experimental design planning with our scientific team.
Optimization of assay conditions, positive controls, and detection parameters for your specific enzyme system.
High-quality kinetic data acquisition with appropriate replicates and statistical rigor.
Comprehensive data analysis, parameter fitting, and detailed report preparation with expert interpretation.
Our team stays at the forefront of enzyme characterization methodology.
Wang, S., Breslawec, A. P., & Poulin, M. B. (2022). Multifunctional fluorescent probes for high-throughput characterization of hexosaminidase enzyme activity. Bioorganic Chemistry, 119, 105532.
Shortall, K., Magner, E., & Soulimane, T. (2022). Expression, purification, and in vitro enzyme activity assay of a recombinant aldehyde dehydrogenase from Thermus thermophilus, using an Escherichia coli host. Bio Protoc, 12(9), e4401.
Simon-Baram, H., Roth, S., Niedermayer, C., et al. (2022). A high-throughput continuous spectroscopic assay to measure the activity of natural product methyltransferases. Chembiochem, 23(17), e202200162.
Beech, J. L., Clare, R., Kincannon, W. M., et al. (2022). A flexible kinetic assay efficiently sorts prospective biocatalysts for PET plastic subunit hydrolysis. RSC Advances, 12(13), 8119–8130.
Legare, S., Heide, F., Bailey-Elkin, B. A., & Stetefeld, J. (2022). Improved SARS-CoV-2 main protease high-throughput screening assay using a 5-carboxyfluorescein substrate. Journal of Biological Chemistry, 298(4), 101739.
We proudly support academic and industrial research organizations worldwide.
Find answers to common questions about our enzyme activity assay services.
We characterize all major enzyme classes including hydrolases, transferases, oxidoreductases, lyases, isomerases, and ligases. Our platform handles proteases, lipases, kinases, phosphatases, glycosidases, and many more enzyme types from various sources.
We offer continuous monitoring assays (fluorescence/absorbance), endpoint assays (HPLC/LC-MS), and discontinuous time-point assays. High-throughput 96-well and 384-well formats are available for rapid screening applications.
All assays include appropriate positive and negative controls, replicate measurements, and statistical analysis. We follow strict SOPs and can accommodate GLP documentation requirements for regulatory studies.
Standard kinetics profiling completes in 1-2 weeks. Comprehensive characterization studies typically require 3-4 weeks. Rush services are available upon request for time-critical projects.
Yes, we offer inhibitor screening against your target enzyme including FDA-approved drug libraries, custom compound collections, and natural product extracts. IC50 determination and selectivity profiling against off-target enzymes are included.
Partner with CD Biosynsis for accurate, reliable enzyme activity data that advances your research programs.
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