From gene to purified protein — high-throughput expression platforms, six expression host systems, and automated purification pipelines. Whether you need soluble E. coli protein or mammalian-cell-produced therapeutics, we have the expertise to deliver.
Six established expression platforms covering bacteria, yeast, insect, mammalian, and cell-free systems. We match your protein to the optimal host based on your application requirements.
Fast, cost-effective expression for soluble proteins, inclusion bodies, and small-to-medium scale production. Multiple strains available.
Pichia pastoris and Saccharomyces cerevisiae for secreted expression with eukaryotic post-translational modifications.
Baculovirus-mediated expression in Sf9, Sf21, and Hi5 cells for complex eukaryotic proteins.
HEK293 and CHO systems for therapeutic-grade proteins with authentic human glycosylation.
GRAS-status Gram-positive host for high-level secreted expression of industrially relevant enzymes.
Purer E. coli cell-free extracts for protein labeling, membrane proteins, and toxic protein expression.
Systematic approach from gene design to purified protein, with screening at every step
We start with your sequence or gene template. Services include gene synthesis with codon optimization, sub-cloning into expression vectors, and sequence verification.
96-well format expression screening tests multiple strains, temperatures, induction times, and media to identify optimal expression conditions before scale-up.
Based on screening results, we scale up in shake flasks or bioreactors. Includes monitoring of growth, induction optimization, and harvest timing to maximize yield.
Automated purification using affinity chromatography, ion exchange, and SEC. All proteins undergo SDS-PAGE analysis and endotoxin testing where applicable.
96-well format screening tests up to 48 conditions simultaneously. Identify the best expression conditions in days, not weeks, before committing to large-scale production.
Standard expression projects complete in 3-4 weeks. Our established protocols for common tags and expression systems enable fast turnaround without sacrificing quality.
We have extensive experience with membrane proteins, multi-domain complexes, kinases, and proteins with disulfide bonds. Our screening approach finds what works for your specific target.
For E. coli expressed proteins, we offer different endotoxin specifications through additional purification steps. Endotoxin testing is included in our standard QC package.
Our expression platform builds on peer-reviewed methods published in leading journals
Ingram, Z. et al. Overcoming Obstacles in Protein Expression in the Yeast Pichia pastoris: Interviews of Leaders in the Pichia Field. Biology 11, 1434 (2022). https://pubmed.ncbi.nlm.nih.gov/36213698/
Interviews with leading researchers covering common challenges and best practices in Pichia pastoris expression systems.Tan, E. et al. HEK293 Cell Line as a Platform to Produce Recombinant Proteins and Viral Vectors. Front Bioeng Biotechnol 9, 796991 (2021). https://pubmed.ncbi.nlm.nih.gov/34966729/
Comprehensive review of HEK293 cells for recombinant protein and viral vector production including comparison with other mammalian systems.Völzke, J.L. et al. Efficient Purification of Polyhistidine-Tagged Recombinant Proteins Using Functionalized Corundum Particles. BioTech 12, 31 (2023). https://pubmed.ncbi.nlm.nih.gov/37218748/
Advanced IMAC purification methods for His-tagged proteins, including optimized bind-wash-elute strategies.Standard delivery includes quality-verified purity. Higher purity levels are available through additional chromatography steps. For structural biology, we offer gel-filtration polishing to remove aggregates and deliver monodisperse protein.
Yes. We have extensive experience with membrane proteins, multi-domain complexes, kinases, and proteins with disulfide bonds. Our screening approach systematically tests multiple conditions to find what works for your specific target.
For E. coli expressed proteins, we offer different endotoxin specifications through additional purification steps. Endotoxin testing is included in our standard QC package, with reduced levels available upon request.
Yes. Standard characterization includes SDS-PAGE, mass spectrometry (MW confirmation), and concentration determination. Additional services include circular dichroism (CD), thermal stability (DSF), SEC-MALS for aggregation, and binding assays.
The choice depends on your protein type and application. E. coli is fastest and most cost-effective for simple proteins. Yeast offers eukaryotic modifications and secreted expression. Mammalian cells are best for therapeutic proteins requiring authentic human glycosylation. Contact us to discuss your specific requirements.
Contact us with your protein requirements for a custom quote and project timeline. Our team will recommend the optimal expression strategy.
Tell us about your project and we'll get back within 24 hours.
