We deliver research-grade and pharmaceutical-grade purified proteins using state-of-the-art chromatography platforms. From initial sample assessment to final QC, our workflows are designed to meet your purity, yield, and timeline requirements.
We offer end-to-end protein purification services, from quick analytical-scale runs to large-scale production. Every project is supported by rigorous quality control to ensure you receive proteins that meet your functional and structural requirements.
Fast-track screening of purification strategies using high-throughput chromatography platforms. We identify optimal conditions within days to meet aggressive project timelines.
We handle challenging targets including membrane proteins, multi-subunit complexes, and post-translationally modified proteins that require specialized protocols.
Dedicated endotoxin removal and testing workflows to achieve pharmaceutical-grade purity. We routinely deliver proteins with endotoxin levels below 0.1 EU/mg.
From milligram analytical batches to multi-gram manufacturing runs, our purification pipelines scale seamlessly with your project requirements and budget.
Our chromatography infrastructure supports every major protein purification strategy, enabling us to design the optimal workflow for your specific target.
Primary capture step for His-tagged proteins using Ni-NTA and Co-TALON resins. We optimize imidazole gradients and resin selection for maximum yield and purity in a single step.
High-resolution separation based on surface charge. We employ both cation and anion exchange formats to polish proteins to >98% purity and remove trace contaminants.
Separation based on surface hydrophobicity using HIC resins and multimodal ligands. Effective for removing aggregates and isoforms that co-purify with conventional methods.
Final polishing step for buffer exchange, desalt, and removal of aggregates and fragments. We operate analytical, preparative, and HiLoad SEC columns across multiple resin formats.
Specialized workflows for therapeutic and in-vivo applications. We combine polymyxin B columns, Triton X-114 extraction, and anion exchange to achieve endotoxin levels below detection thresholds.
From milligram to multi-gram batches using ÄKTA process and pilot systems. We design scalable protocols that maintain product quality from analytical runs through manufacturing scale.
Structured quality-controlled pipeline from initial sample receipt through final delivery
We analyze your expression lysate for target abundance, solubility, and complexity to design the most efficient purification strategy.
Primary capture using affinity or ion exchange chromatography to rapidly enrich the target protein from the crude lysate.
Hydrophobic interaction, multimodal, or ion exchange chromatography to remove bulk contaminants and close purity gaps.
Size exclusion chromatography for buffer exchange and aggregate removal, followed by SDS-PAGE, SEC-MALS, and endotoxin QC.
Expertise across diverse protein classes and therapeutic development contexts
Purification of functional G-protein coupled receptors from insect cell and mammalian expression systems using detergent-based extraction and streptavidin affinity chromatography.
Purpose-built purification workflows for bispecific antibodies, Fab fragments, and scFv constructs from mammalian expression systems with rigorous aggregate control.
Purification of thermally stable enzyme complexes from thermophilic expression hosts, including cellulases, amylases, and multipartite enzyme assemblies for industrial biocatalysis.
Our purification workflows are grounded in peer-reviewed methods from leading journals
Du, M., Hou, Z., Liu, L., Xuan, Y., Chen, X., Fan, L., Li, Z. and Xu, B. Progress, applications, challenges and prospects of protein purification technology. Front Bioeng Biotechnol 10, 1028691 (2022). https://pubmed.ncbi.nlm.nih.gov/36561042/
Comprehensive review of current strategies for recombinant protein purification, including affinity tags, chromatography methods, and emerging technologies.Kielkopf, C.L., Bauer, W. and Urbatsch, I.L. Purification of Polyhistidine-Tagged Proteins by Immobilized Metal Affinity Chromatography. Cold Spring Harb Protoc 2020, (2020). https://pubmed.ncbi.nlm.nih.gov/32482902/
Standard protocol for IMAC purification of His-tagged proteins, covering resin preparation, binding, washing, elution, and resin regeneration.Lau, E.C.H.T., Dodds, K.C., McKenna, C., Cowan, R.M., Ganin, A.Y., Campopiano, D.J. and Yiu, H.H.P. Direct purification and immobilization of his-tagged enzymes using unmodified nickel ferrite NiFe2O4 magnetic nanoparticles. Sci Rep 13, 21549 (2023). https://pubmed.ncbi.nlm.nih.gov/38057439/
Magnetic nanoparticle-based IMAC using unmodified NiFe2O4 for rapid, scalable one-step purification and immobilization of His-tagged enzymes.We offer IMAC (Ni-NTA, Co-TALON), strong/weak cation exchange (SP, CM), strong/weak anion exchange (Q, DEAE), hydrophobic interaction chromatography (HIC), multimodal chromatography (Capto MMC), and size exclusion chromatography (SEC). Our workflows combine multiple techniques to achieve your target purity level.
We routinely achieve >95% purity for standard proteins and >98% for applications requiring higher purity such as structural biology or therapeutic development. Endotoxin levels can be reduced to <0.1 EU/mg upon request. Actual purity is confirmed by SDS-PAGE and SEC-MALS before delivery.
We purify proteins from all major expression platforms: E. coli, Pichia pastoris, Saccharomyces cerevisiae, insect cells (baculovirus), and mammalian cells (HEK293, CHO). Each system has specific considerations for downstream purification, and we account for these in our workflow design.
Standard purification projects complete in 2-3 weeks from protein delivery. Complex projects involving challenging targets, membrane proteins, or endotoxin removal may require 4-6 weeks. We provide a project-specific timeline after initial sample assessment.
Yes. We provide dedicated endotoxin removal using polymyxin B columns, Triton X-114 extraction, and anion exchange chromatography. We can achieve endotoxin levels below 0.1 EU/mg for in-vivo and therapeutic applications, with LAL or PyroGene assay documentation.
Our team designs custom purification workflows for every project. Share your protein details and purity requirements, and we'll respond within one business day.
Tell us about your project and we'll get back within 24 hours.
