From shake flask to industrial-scale bioreactor — comprehensive process development, optimization, and scale-up for recombinant protein and metabolite production.
Our bioprocess development laboratory combines shake flask studies with multi-scale bioreactor systems to deliver optimized fermentation processes for any application.
Systematic optimization of fermentation conditions including media composition, temperature, pH, dissolved oxygen, and feeding strategies.
Seamless scale-up from shake flask through bench-top bioreactor to pilot scale with consistent performance and quality attributes.
Integrated downstream development including cell disruption, clarification, chromatography, and formulation for complete process integration.
From microbial fermentation to mammalian cell culture, our platform covers all aspects of bioprocess development.
Bacterial and yeast fermentation platforms optimized for high cell density and maximum product titer.
Mammalian and insect cell culture platforms for recombinant protein and virus production.
Real-time monitoring of critical process parameters including dissolved oxygen, pH, temperature, and metabolites.
Comprehensive analytical support for product quantification, purity assessment, and quality control.
Our bioprocess development expertise combines state-of-the-art equipment with proven methodologies to deliver optimized processes efficiently.
Bioreactors from 0.5L to 50L working volume for seamless scale-up from development to pilot production.
Design of experiments approach for systematic, statistically-rigorous process optimization.
Continuous monitoring of critical process parameters with automated data collection and reporting.
Dedicated process development scientists with extensive experience in microbial and cell culture systems.
Our fermentation services support production across multiple industries and product classes.
High-titer production of therapeutic proteins using optimized microbial and mammalian cell culture platforms. From enzyme candidates to antibody fragments.
Cost-effective production of industrial enzymes including lipases, cellulases, and amylases for detergent, food, and biofuel applications.
Production of microbial metabolites, amino acids, vitamins, and other small molecules through optimized fermentation processes.
Our proven workflow ensures efficient project delivery from initial development to pilot-scale demonstration.
Project scope discussion, host selection, and process strategy development with our bioprocess team.
Shake flask studies and bioreactor optimization for media composition and process parameters.
Progressive scale-up with consistent quality attributes and process characterization.
Documentation package preparation and technology transfer to manufacturing partners.
Our team stays at the forefront of bioprocess development methodology.
Li, C., Xu, D., Xiong, Z., et al. (2022). Optimization of the fermentative production of Rhizomucor miehei lipase in Aspergillus oryzae by controlling morphology. Bioengineering, 9(11), 610.
Liu, S., Liu, H., Zhou, L., et al. (2022). Enhancement of antibacterial and growth-promoting effects of Paenibacillus polymyxa by optimizing its fermentation process. Journal of Applied Microbiology, 133(5), 2954–2965.
Teng, X., Li, C., Yi, X., et al. (2021). A novel scale-up strategy for cultivation of BHK-21 cells based on similar hydrodynamic environments in the bioreactors. Bioresources and Bioprocessing, 8(1), 74.
Premalatha, A., Vijayalakshmi, K., Shanmugavel, M., & Rajakumar, G. S. (2023). Optimization of culture conditions for enhanced production of extracellular alpha-amylase using solid-state and submerged fermentation from Aspergillus tamarii. Biotechnology and Applied Biochemistry, 70(2), 835–845.
Saleena, S. K., Johnson, J. I., Joseph, J. K., et al. (2023). Production and optimization of L-asparaginase by Streptomyces koyangensis SK4 isolated from Arctic sediment. Journal of Basic Microbiology, 63(3-4), 417–426.
Find answers to common questions about our fermentation optimization services.
We support all common microbial and cell culture hosts: E. coli, Pichia pastoris, Bacillus subtilis, Saccharomyces cerevisiae, CHO cells, HEK293 cells, and insect cells. Each host has specific requirements that we account for in process development.
We offer bioreactors from 0.5L to 50L working volume. This range supports process development through pilot-scale production suitable for early clinical trial material.
Scale-up follows established principles maintaining similar oxygen transfer rates, shear conditions, and process trajectories across scales. We characterize processes at bench scale and translate parameters to larger vessels while monitoring critical quality attributes.
We use DoE-based approaches to systematically optimize key variables including media composition, feeding strategies, and process parameters. Typical improvements vary depending on the starting point and biological system.
Our 50L bioreactor capacity is suitable for clinical trial material (Phase I/II) production. We operate under quality systems appropriate for early-stage clinical supply. For later-stage production, we assist with technology transfer to GMP manufacturing partners.
Partner with CD Biosynsis for efficient bioprocess development that accelerates your path to production.
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